Rolling Circle Amplification (RCA)

This book covers the latest developments in rolling circle amplification (RCA) technology with applications in clinical diagnostic tests and molecular medicine. Topics covered include new enzymes useful in RCA, the RCA-involving techniques for enhanced signal amplification, novel RCA diagnostics, sensors for expedite RCA detection, and prospective RCA-based therapeutics. This is a valuable book for university professors and students in the field of biomedical engineering and biomolecular pharmacology as well as R&D managers of biotechnology and biopharmaceutical companies. Specifically, this book: Reviews prospective RCAbased therapeutics, including RCAderived DNA nanoparticles that strongly bind to cancer cells, along with DNA nanoclews and DNA origami nanostructrures as vehicles for intracellular delivery of various drugs Expands readers' understanding of sensor systems for expediting detection of RCA products by using probetagged magnetic nanobeads Maximizes reader insights into novel RCA diagnostics, such as PNA openersassisted RCA for detection of single target cells, ramified RCA for identifying the diseaserelated SNPs, RNAprimed RCA for detection of microbial pathogens and in situ RCA diagnosis of cancer cells and malignant tissues Presents innovative methods for quasiexponential enhancement of RCAgenerated signals, such as nicking enzymeassisted cascade RCA and RCA coupled with loopmediated amplification Advance Praise for Rolling Circle Amplification (RCA): "This book provides a badly needed compendium of innovative RCA methods and applications. It should help further increase the community of scientists that have employed RCA in research and diagnostic programs."- Charles Cantor, Professor Emeritus of Biomedical Engineering, Boston University Executive Director, Retrotope Inc. (USA) "In this new book Vadim Demidov has assembled an enticing menu of articles that illustrate the evolution of the RCA field, including improved protein parts for building superior DNA nanomachines, enhanced modalities of amplification and detection, diagnostic applications, and even a sampling of potential therapeutic applications. The reader will appreciate that while RCA has come of age, there is no lack of exciting surprises, turns, and twists in the continuing evolution of the technology."- Paul Lizardi, Professor of Pathology, Yale University School of Medicine (retired) Investigator, University of Granada, Spain, President, PetaOmics, Inc., San Marcos, Texas "The rolling circle concept for isothermal amplification is appealingly simple and has been adopted widely. This timely book details some of the promising applications under development that the technology makes possible."- Eric Kool, George and Hilda Daubert Professor of Chemistry, Stanford University, USA "Rolling circle amplification offers some unique advantages in molecular biotechnology and molecular medicine. This book is a valuable occasion to illustrate the range of opportunities this creates before a wide readership, hopefully stimulating more to take advantage of this exciting technology."- Ulf Landegren, Professor of Molecular Medicine, Uppsala University, Sweden

Vadim V. Demidov, Ph.D., is a Senior Analyst in the Biotechnology & Pharmaceuticals Group at Global Prior Art, Inc. (Boston, USA), an intellectual property research and analysis firm. Dr. Demidov received his M.S. degree in Physical and Chemical Engineering from Moscow Institute of Physics and Technology (MIPT, a leading Russian technical university, also known as "the Russian MIT") and his Ph.D. degree in Biophysics from the Institute of Molecular Genetics of the Russian Academy of Sciences (IMGRAS) and MIPT. Before joining the Global Prior Art company in 2008, he worked for almost 30 years in academia and industry worldwide. Dr. Demidov is well known in the molecular biology and biotechnology field for his innovative studies related to peptide nucleic acid (PNA). He has introduced the use of PNA openers for detection of specific sequences within DNA duplexes under non-denaturing conditions, with some of these applications involving rolling circle amplification (RCA).